Neutralizing Antibody Test Instructions

Antigen Test Instructions

Ampoule Buffer Mode

Bottle Buffer Mode

A. For fingerstick blood: Wipe the complete fingertip of subject’s middle(3rd) or ring(4th) finger with the alcohol wipe or swab, wait until the fingertip has completely dried (minimum 30 seconds); Punch the fingertip with safety lancet and make sure a well-formed drop of blood is present: Use the capillary pipet to draw sample up to the pipet’s black line indicator.

B. For serum: Take up 5 μL of serum sample by volumetric pipettor.

Release the sample to the “S1” area indicated by arrow mark.

Release the sample to the “S1” area indicated by arrow mark.

Twist open the provided extraction buffer ampoule at upright position, with notched neck side on top. Squeeze the ampoule to empty extraction buffer into well area marked as “S”.

Add 2 drops of sample buffer to sample well marked as “S”.

High Level NAb

In addition to the control band, if the color of test band is equal or stronger than that of reference band, it indicates the neutralizing antibody level is at high level.

Low Level NAb

In addition to the control band, if the color intensity of the test band is less than that of reference band, it indicates the neutralizing antibody level is low.

Negative

In addition to the control band, if only a colored reference band appears, it indicates no neutralizing antibody.

Invalid

Invalid

Invalid

The test result is invalid if a colored band does not form in the control region. The sample must be re-tested, using a new test device.

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